The Antigen-Antibody Reaction in Immunohistochemistry

Factors affecting the antigen-antibody reaction.

Types of weak interactions According to quantum mechanics, all chemical bonds are based on electrostatic forces. A list of types of weak bonds is shown in table I, together with their strength (energy). Van der Waals forces are the weakest, but they are able to attract all kinds of molecules. Hydrogen or iondipole bonds require oppositely charged atoms. Hydrogen bonds are very important in aque...

The antibody-antigen reaction: a visual observation.

The Cutaneous Reaction to Soluble Antigen-antibody Complexes

The in vivo activity of soluble antigen-antibody complexes was tested by a single intradermal injection in rabbits. Skin reactions were obtained marked by erythema, induration, and occasionally hemorrhage and necrosis. Microscopically, diffuse inflammation and occasional vascular necrosis could be found at all dosages. This indicates that soluble antigen-antibody complexes are phlogogenic and p...

Biochemical Study of Cellular Antigen-antibody Reaction in Tissue Culture

The correlation between morphological and biochemical changes produced by the antigen-antibody reaction was studied in cultures of tissue monocytes taken from sensitized animals. The cells were grown under conditions which allowed collection of samples from the culture fluid as well as microscopic observation. Introduction of the antigen into the culture medium causes rapid release of a proteas...

The antigen-antibody reaction. I. The influence of sodium chloride concentration on the quantitative precipitin reaction.